Journal: Frontiers in Veterinary Science

Article Title: Detection of bimodal survivin expressions in canine cancer types by flow cytometry compared to immunohistochemistry

doi: 10.3389/fvets.2025.1552415

Figure Lengend Snippet: Analysis of survivin expression sites by IHC and WB. (A) Images of HE staining and survivin expression via IHC in canine melanoma cell lines (CMM2, CMeC, and LMeC) are shown. (B) Images of HE staining and survivin expression via IHC in murine cell lines (p815, CT26, and B16F10) are shown. (C) The cytosol and total survivin expression patterns via WB are indicated. (D) The expression levels of total survivin (blue bar) and cytosolic survivin (orange bar) corrected on the basis of β-actin expression are shown via density of plot analysis via ImageJ software.

Article Snippet: A total of six cell lines were used: canine malignant melanoma lines [CMM2, CMeC2, LMeC; provided by Dr. Takayuki Nakagawa, Department of Veterinary Surgery, University of Tokyo; ( )], the murine malignant melanoma line B16F10, the murine mast cell tumor line p815, and the murine colon cancer line CT26 (distributed for a fee by the JCRB Cell Bank).

Techniques: Expressing, Staining, Software

Journal: Clinical Cancer Research

Article Title: Tumor Microenvironment Remodeling by Intratumoral Oncolytic Vaccinia Virus Enhances the Efficacy of Immune-Checkpoint Blockade

doi: 10.1158/1078-0432.ccr-18-1932

Figure Lengend Snippet: Figure 2. Intratumoral injection of JX leads to systemic and cancer-specific immune responses. Mice were s.c. injected with Renca tumor cells in the right flank and with Renca or CT26 tumors in the left flank. Arrows indicated intratumoral JX treatment. A, Growth curves of JX-injected Renca tumor and noninjected Renca tumor. B, Representative images and comparisons of CD8þ T cells in the JX-injected and noninjected tumors. C, Growth curve of JX-injected Renca tumor and noninjected CT26 tumor. D, Representative images and comparisons of CD8þ T cells in the JX-injected and noninjected tumors. Unless otherwise denoted, n ¼ 5 for each group. Values are mean SD. , P < 0.05 versus control. ns, not significant. Two-tailed Student t test was used. Scale bars, 50 mm.

Article Snippet: The Renca murine renal cancer cell line and the CT26 murine colon cancer cell line were obtained from the ATCC (#CRL-2947) and Korean Cell LineBank (#80009).

Techniques: Injection, Control, Two Tailed Test

Journal: Clinical Cancer Research

Article Title: Tumor Microenvironment Remodeling by Intratumoral Oncolytic Vaccinia Virus Enhances the Efficacy of Immune-Checkpoint Blockade

doi: 10.1158/1078-0432.ccr-18-1932

Figure Lengend Snippet: Figure 5. The triple combination of JX and aPD-1 and aCTLA-4 induces profound tumor regression and provides a long-term survival benefit in kidney cancer. Mice were s.c. implanted with Renca tumors and treated with or without JX and immune-checkpoint blockades for PD-1 and CTLA-4 on the indicated days (arrows). A, Comparisons of tumor growth. Mean and individual tumor growth curves over time. Pooled data from two experiments with 8 animals per group. , P < 0.05 versus control; #, P < 0.05 versus JX; $, P < 0.05 versus aPD-1þ aCTLA-4. ns, not significant. Two-tailed Student t test was used. B, Waterfall plot showing the maximal percent changes from baseline in tumor size. C, Comparison of tumor size after injection of Renca or CT26 tumor cells into mice with complete tumor regression or into na€ve mice. D, Kaplan–Meier plot for overall survival. n ¼ 8–11 for each group. Log-rank test was used.

Article Snippet: The Renca murine renal cancer cell line and the CT26 murine colon cancer cell line were obtained from the ATCC (#CRL-2947) and Korean Cell LineBank (#80009).

Techniques: Control, Two Tailed Test, Comparison, Injection